A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms

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A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms.

Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction in cheese production. Quicker curd contraction reduce...

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Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of mismatch bases. The optimal selection of primers can be achieved in an automated way using a program which evaluates candidate primers for a given...

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ژورنال

عنوان ژورنال: Genetics and Molecular Research

سال: 2013

ISSN: 1676-5680

DOI: 10.4238/2013.december.11.3